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https://www.bioz.com/product/lfd/lfd+mice+perio/pm42286074-107-29-29
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Screening of the optimal primer pair for RT-ERA and determination of the optimal <t>RT-ERA–LFD</t> probe. (A) Screening of BRSV RT-ERA primer pairs. M: DNA marker DL2000; 1–12: The amplification results of 12 primer pairs BRSV-ERA-F1/R1 to BRSV-ERA-F3/R4; 13–24: Negative controls for amplification with 12 primer pairs BRSV-ERA-F1/R1–BRSV-ERA-F3/R4. (B) Screening of BRSV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4; 4–6: Negative controls for amplification with primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4. (C) Screening of BVDV RT-ERA primer pairs. M: DNA marker DL2000; 1–8: Amplification results of 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4; 9–16: Negative controls for amplification with 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4. (D) Screening of BVDV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4; 4–6: Negative controls for amplification with primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4. (E) Screening of the optimal probe for BRSV RT-ERA-LFD. 1: BRSV-ERA-P1; 2: BRSV-ERA-P2; 3: Negative control for BRSV-ERA-P1; 4: Negative control for BRSV-ERA-P2. (F) Screening of the optimal probe for BVDV RT-ERA-LFD. 1: BVDV-ERA-P1; 2: BVDV-ERA-P2; 3: BVDV-ERA-P3; 4: Negative control for BVDV-ERA-P1; 5: Negative control for BVDV-ERA-P2; 6: Negative control for BVDV-ERA-P3.
Era Lfd Assay, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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era lfd assay - by Bioz Stars, 2026-09
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Screening of the optimal primer pair for RT-ERA and determination of the optimal <t>RT-ERA–LFD</t> probe. (A) Screening of BRSV RT-ERA primer pairs. M: DNA marker DL2000; 1–12: The amplification results of 12 primer pairs BRSV-ERA-F1/R1 to BRSV-ERA-F3/R4; 13–24: Negative controls for amplification with 12 primer pairs BRSV-ERA-F1/R1–BRSV-ERA-F3/R4. (B) Screening of BRSV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4; 4–6: Negative controls for amplification with primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4. (C) Screening of BVDV RT-ERA primer pairs. M: DNA marker DL2000; 1–8: Amplification results of 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4; 9–16: Negative controls for amplification with 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4. (D) Screening of BVDV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4; 4–6: Negative controls for amplification with primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4. (E) Screening of the optimal probe for BRSV RT-ERA-LFD. 1: BRSV-ERA-P1; 2: BRSV-ERA-P2; 3: Negative control for BRSV-ERA-P1; 4: Negative control for BRSV-ERA-P2. (F) Screening of the optimal probe for BVDV RT-ERA-LFD. 1: BVDV-ERA-P1; 2: BVDV-ERA-P2; 3: BVDV-ERA-P3; 4: Negative control for BVDV-ERA-P1; 5: Negative control for BVDV-ERA-P2; 6: Negative control for BVDV-ERA-P3.
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Screening of the optimal primer pair for RT-ERA and determination of the optimal <t>RT-ERA–LFD</t> probe. (A) Screening of BRSV RT-ERA primer pairs. M: DNA marker DL2000; 1–12: The amplification results of 12 primer pairs BRSV-ERA-F1/R1 to BRSV-ERA-F3/R4; 13–24: Negative controls for amplification with 12 primer pairs BRSV-ERA-F1/R1–BRSV-ERA-F3/R4. (B) Screening of BRSV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4; 4–6: Negative controls for amplification with primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4. (C) Screening of BVDV RT-ERA primer pairs. M: DNA marker DL2000; 1–8: Amplification results of 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4; 9–16: Negative controls for amplification with 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4. (D) Screening of BVDV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4; 4–6: Negative controls for amplification with primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4. (E) Screening of the optimal probe for BRSV RT-ERA-LFD. 1: BRSV-ERA-P1; 2: BRSV-ERA-P2; 3: Negative control for BRSV-ERA-P1; 4: Negative control for BRSV-ERA-P2. (F) Screening of the optimal probe for BVDV RT-ERA-LFD. 1: BVDV-ERA-P1; 2: BVDV-ERA-P2; 3: BVDV-ERA-P3; 4: Negative control for BVDV-ERA-P1; 5: Negative control for BVDV-ERA-P2; 6: Negative control for BVDV-ERA-P3.
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Screening of the optimal primer pair for RT-ERA and determination of the optimal <t>RT-ERA–LFD</t> probe. (A) Screening of BRSV RT-ERA primer pairs. M: DNA marker DL2000; 1–12: The amplification results of 12 primer pairs BRSV-ERA-F1/R1 to BRSV-ERA-F3/R4; 13–24: Negative controls for amplification with 12 primer pairs BRSV-ERA-F1/R1–BRSV-ERA-F3/R4. (B) Screening of BRSV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4; 4–6: Negative controls for amplification with primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4. (C) Screening of BVDV RT-ERA primer pairs. M: DNA marker DL2000; 1–8: Amplification results of 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4; 9–16: Negative controls for amplification with 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4. (D) Screening of BVDV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4; 4–6: Negative controls for amplification with primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4. (E) Screening of the optimal probe for BRSV RT-ERA-LFD. 1: BRSV-ERA-P1; 2: BRSV-ERA-P2; 3: Negative control for BRSV-ERA-P1; 4: Negative control for BRSV-ERA-P2. (F) Screening of the optimal probe for BVDV RT-ERA-LFD. 1: BVDV-ERA-P1; 2: BVDV-ERA-P2; 3: BVDV-ERA-P3; 4: Negative control for BVDV-ERA-P1; 5: Negative control for BVDV-ERA-P2; 6: Negative control for BVDV-ERA-P3.
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Average 86 stars, based on 1 article reviews
lfd - by Bioz Stars, 2026-09
86/100 stars
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Screening of the optimal primer pair for RT-ERA and determination of the optimal RT-ERA–LFD probe. (A) Screening of BRSV RT-ERA primer pairs. M: DNA marker DL2000; 1–12: The amplification results of 12 primer pairs BRSV-ERA-F1/R1 to BRSV-ERA-F3/R4; 13–24: Negative controls for amplification with 12 primer pairs BRSV-ERA-F1/R1–BRSV-ERA-F3/R4. (B) Screening of BRSV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4; 4–6: Negative controls for amplification with primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4. (C) Screening of BVDV RT-ERA primer pairs. M: DNA marker DL2000; 1–8: Amplification results of 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4; 9–16: Negative controls for amplification with 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4. (D) Screening of BVDV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4; 4–6: Negative controls for amplification with primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4. (E) Screening of the optimal probe for BRSV RT-ERA-LFD. 1: BRSV-ERA-P1; 2: BRSV-ERA-P2; 3: Negative control for BRSV-ERA-P1; 4: Negative control for BRSV-ERA-P2. (F) Screening of the optimal probe for BVDV RT-ERA-LFD. 1: BVDV-ERA-P1; 2: BVDV-ERA-P2; 3: BVDV-ERA-P3; 4: Negative control for BVDV-ERA-P1; 5: Negative control for BVDV-ERA-P2; 6: Negative control for BVDV-ERA-P3.

Journal: Frontiers in Veterinary Science

Article Title: Establishment and field validation of a rapid on-site recombinase polymerase amplification–lateral flow assay for BRSV and BVDV

doi: 10.3389/fvets.2026.1754704

Figure Lengend Snippet: Screening of the optimal primer pair for RT-ERA and determination of the optimal RT-ERA–LFD probe. (A) Screening of BRSV RT-ERA primer pairs. M: DNA marker DL2000; 1–12: The amplification results of 12 primer pairs BRSV-ERA-F1/R1 to BRSV-ERA-F3/R4; 13–24: Negative controls for amplification with 12 primer pairs BRSV-ERA-F1/R1–BRSV-ERA-F3/R4. (B) Screening of BRSV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4; 4–6: Negative controls for amplification with primer pairs BRSV-ERA-F1/R4, BRSV-ERA-F2/R4, and BRSV-ERA-F3/R4. (C) Screening of BVDV RT-ERA primer pairs. M: DNA marker DL2000; 1–8: Amplification results of 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4; 9–16: Negative controls for amplification with 8 primer pairs BVDV-ERA-F1/R1–BVDV-ERA-F2/R4. (D) Screening of BVDV RT-ERA optimal primer pair. M: DNA marker DL2000; 1–3: Amplification results of primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4; 4–6: Negative controls for amplification with primer pairs BVDV-ERA-F1/R2, BVDV-ERA-F1/R3, and BVDV-ERA-F1/R4. (E) Screening of the optimal probe for BRSV RT-ERA-LFD. 1: BRSV-ERA-P1; 2: BRSV-ERA-P2; 3: Negative control for BRSV-ERA-P1; 4: Negative control for BRSV-ERA-P2. (F) Screening of the optimal probe for BVDV RT-ERA-LFD. 1: BVDV-ERA-P1; 2: BVDV-ERA-P2; 3: BVDV-ERA-P3; 4: Negative control for BVDV-ERA-P1; 5: Negative control for BVDV-ERA-P2; 6: Negative control for BVDV-ERA-P3.

Article Snippet: All amplicons that gave a positive signal in the ERA-LFD assay were sent to Sangon Biotech for sequencing to confirm their identity and accuracy.

Techniques: Marker, Amplification, Negative Control

Optimization of the BRSV and BVDV RT-ERA-LFD platform conditions. (A,B) Optimization of the RT-ERA-LFD reaction temperature for BRSV and BVDV. Temperature gradients of 39 °C, 40 °C, 41 °C, 42 °C, and 43 °C were tested for BRSV (A) and BVDV (B) , with corresponding negative controls. (C,D) Optimization of the RT-ERA-LFD reaction time for BRSV and BVDV. Time gradients of 10, 15, 20, 25, 30, 35 and 40 min were tested for BRSV (C) and BVDV (D) , with corresponding negative controls.

Journal: Frontiers in Veterinary Science

Article Title: Establishment and field validation of a rapid on-site recombinase polymerase amplification–lateral flow assay for BRSV and BVDV

doi: 10.3389/fvets.2026.1754704

Figure Lengend Snippet: Optimization of the BRSV and BVDV RT-ERA-LFD platform conditions. (A,B) Optimization of the RT-ERA-LFD reaction temperature for BRSV and BVDV. Temperature gradients of 39 °C, 40 °C, 41 °C, 42 °C, and 43 °C were tested for BRSV (A) and BVDV (B) , with corresponding negative controls. (C,D) Optimization of the RT-ERA-LFD reaction time for BRSV and BVDV. Time gradients of 10, 15, 20, 25, 30, 35 and 40 min were tested for BRSV (C) and BVDV (D) , with corresponding negative controls.

Article Snippet: All amplicons that gave a positive signal in the ERA-LFD assay were sent to Sangon Biotech for sequencing to confirm their identity and accuracy.

Techniques:

Evaluation of sensitivity, specificity, and concordance of the detection platform. (A,B) Specificity evaluation of BRSV and BVDV RT-ERA-LFD assays. Genomic materials of BRSV, BVDV, IBRV, BCoV, P. multocida and T. gondii archived in our laboratory were used for specificity testing. In (A) , lanes 1–7 contain BRSV, IBRV, BVDV, BCoV, P. multocida , T. gondii and a negative control; in (B) the order is BVDV, IBRV, BRSV, BCoV, P. multocida , T. gondii and negative control. (C) Sensitivity evaluation of BRSV RT-ERA-LFD (left) and RT-PCR (right). In the left panel, lanes contain 10 8 –10 0 copies/μL BRSV RNA standard followed by the negative control; in the right panel, the same samples are preceded by the DNA DL2000 marker. (D) Sensitivity evaluation of BVDV RT-ERA-LFD (left) and RT-PCR (right). In the left panel, lanes contain 10 8 –10 0 copies/μL BVDV RNA standard followed by the negative control; in the right panel, the same samples are preceded by the DNA DL2000 marker. (E) Concordance testing of BRSV RT-ERA-LFD (left) versus RT-PCR (right). Left panel: 26 suspected samples plus negative control. Right panel: DNA DL2000 marker, the same 26 suspected samples, and both positive and negative controls. (F) Concordance testing of BVDV RT-ERA-LFD (left) versus RT-PCR (right). Left panel: 26 suspected samples plus negative control. Right panel: DNA DL2000 marker, the same 26 suspected samples, and both positive and negative controls.

Journal: Frontiers in Veterinary Science

Article Title: Establishment and field validation of a rapid on-site recombinase polymerase amplification–lateral flow assay for BRSV and BVDV

doi: 10.3389/fvets.2026.1754704

Figure Lengend Snippet: Evaluation of sensitivity, specificity, and concordance of the detection platform. (A,B) Specificity evaluation of BRSV and BVDV RT-ERA-LFD assays. Genomic materials of BRSV, BVDV, IBRV, BCoV, P. multocida and T. gondii archived in our laboratory were used for specificity testing. In (A) , lanes 1–7 contain BRSV, IBRV, BVDV, BCoV, P. multocida , T. gondii and a negative control; in (B) the order is BVDV, IBRV, BRSV, BCoV, P. multocida , T. gondii and negative control. (C) Sensitivity evaluation of BRSV RT-ERA-LFD (left) and RT-PCR (right). In the left panel, lanes contain 10 8 –10 0 copies/μL BRSV RNA standard followed by the negative control; in the right panel, the same samples are preceded by the DNA DL2000 marker. (D) Sensitivity evaluation of BVDV RT-ERA-LFD (left) and RT-PCR (right). In the left panel, lanes contain 10 8 –10 0 copies/μL BVDV RNA standard followed by the negative control; in the right panel, the same samples are preceded by the DNA DL2000 marker. (E) Concordance testing of BRSV RT-ERA-LFD (left) versus RT-PCR (right). Left panel: 26 suspected samples plus negative control. Right panel: DNA DL2000 marker, the same 26 suspected samples, and both positive and negative controls. (F) Concordance testing of BVDV RT-ERA-LFD (left) versus RT-PCR (right). Left panel: 26 suspected samples plus negative control. Right panel: DNA DL2000 marker, the same 26 suspected samples, and both positive and negative controls.

Article Snippet: All amplicons that gave a positive signal in the ERA-LFD assay were sent to Sangon Biotech for sequencing to confirm their identity and accuracy.

Techniques: Negative Control, Reverse Transcription Polymerase Chain Reaction, Marker

Detection of clinical samples using BRSV RT-ERA-LFD and BVDV RT-ERA-LFD. (A) BRSV RT-ERA-LFD results for 46 clinical samples. (B) qPCR detection of BRSV in the same 46 samples. (C) BVDV RT-ERA-LFD results for the 46 clinical samples. (D) qPCR detection of BVDV in the 46 samples. In each panel, C indicates the positive control and N the negative control.

Journal: Frontiers in Veterinary Science

Article Title: Establishment and field validation of a rapid on-site recombinase polymerase amplification–lateral flow assay for BRSV and BVDV

doi: 10.3389/fvets.2026.1754704

Figure Lengend Snippet: Detection of clinical samples using BRSV RT-ERA-LFD and BVDV RT-ERA-LFD. (A) BRSV RT-ERA-LFD results for 46 clinical samples. (B) qPCR detection of BRSV in the same 46 samples. (C) BVDV RT-ERA-LFD results for the 46 clinical samples. (D) qPCR detection of BVDV in the 46 samples. In each panel, C indicates the positive control and N the negative control.

Article Snippet: All amplicons that gave a positive signal in the ERA-LFD assay were sent to Sangon Biotech for sequencing to confirm their identity and accuracy.

Techniques: Positive Control, Negative Control